How is specificity determined by chymotrypsin?
A) by an interaction of the active site amino acids with the substrate
B) by a binding of the N-terminus amino acid at the active site
C) by a covalent binding of a his residue to the substrate
D) by a conformational change upon the binding of substrate
E) by a binding of the proper amino acid into a deep pocket on the enzyme

Question

Quizplus · Accepted Answer

Chymotrypsin's specificity is determined by the binding of the proper amino acid (typically a large hydrophobic residue like phenylalanine, tryptophan, or tyrosine) into a deep pocket on the enzyme. This pocket accommodates the side chain of the substrate, dictating the specificity of the enzyme.

How Is Specificity Determined by Chymotrypsin