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A Site-Directed Mutagenesis Converting Histidine-57 of Chymotrypsin to a Lysine

Question 42

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A site-directed mutagenesis converting histidine-57 of chymotrypsin to a lysine results in an inactive enzyme even though lysine has an amino group in its side chain. Describe why the scientist may have thought this result surprising and why it wasn't.

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The side group of histidine has two nitr...

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