Reagents necessary for sequencing by chain termination include:
A) template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe.
B) template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase.
C) template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase.
D) All of the above.
E) None of the above

Question

Quizplus · Accepted Answer

The necessary reagents for sequencing by chain termination include template DNA, dNTPs for extension, a primer to start the reaction, dideoxynucleotide analogs (ddNTPs) which act as chain terminators, and DNA polymerase which adds the ddNTPs to the growing chain. A radioactive probe is not necessary as fluorescent dyes are commonly used for detection. RNA polymerase is not used in DNA sequencing.

Reagents Necessary for Sequencing by Chain Termination Include