Coli proteins is responsible for translocation of the mRNA as a result of a conformation change due to the hydrolysis of GTP?
A) RRF
B) EF-Tu
C) EF-G
D) Shine-Delgarno
E) None of the above.

Question

Quizplus · Accepted Answer

EF-G (elongation factor G) is responsible for the translocation of mRNA during protein synthesis in E. coli. It binds to the ribosome after the peptidyl transferase reaction and hydrolyses GTP, causing a conformational change that allows the tRNA carrying the growing polypeptide chain to move from the A-site to the P-site, and the mRNA to move by one codon. RRF (ribosome recycling factor) helps in dissociating the ribosome from the mRNA at the end of translation, while EF-Tu (elongation factor Tu) is involved in the delivery of aminoacyl-tRNAs to the A-site. Shine-Delgarno is a conserved sequence on bacterial mRNA that helps in initiating translation.

Coli Proteins Is Responsible for Translocation of the MRNA as a Result