In E. coli, mismatch repair involves
A) recognition of mismatched pair by MutS.
B) removal of a mismatched nucleotide by DNA polymerase III.
C) cleavage of the backbone by MutH.
D) A and C
E) A, B, and C.

Question

Quizplus · Accepted Answer

Mismatch repair in E. coli involves recognition of a mismatched pair by MutS, followed by methylation-dependent cleavage of the backbone by MutH. The resulting gap is filled in by DNA polymerase III and ligase. Option B, removal of a mismatched nucleotide by DNA polymerase III, is not correct as DNA polymerase III does not have proofreading activity in mismatch repair. Option E is too general and includes incorrect information.

In E. Coli, Mismatch Repair Involves