What do DNA polymerases require for replication to begin?
A) supercoiled DNA
B) single-stranded template DNA
C) a primer strand to elongate
D) All of the above.
E) None of the above.

Question

Quizplus · Accepted Answer

DNA polymerases require a primer strand to elongate. They cannot initiate the synthesis of a new strand of DNA de novo (from scratch). The primer strand provides a 3'-OH group that can be extended by the polymerase. Once elongation begins, the polymerase can synthesize a new strand of DNA using the template strand as a guide. Supercoiled DNA and single-stranded template DNA are not required for replication to begin, although DNA must be unwound and separated into single strands before replication can occur.

What Do DNA Polymerases Require for Replication to Begin