Which of the Following Experiments Would Be Best for Testing

Which of the following experiments would be best for testing the hypothesis that the enzymes did not perform as expected because the temperature was too high?

A) Produce one batch of blue jeans with twice as much enzyme as another batch of blue jeans while keeping the temperature the same, and then compare how the lightness of the blue jeans varies between batches.
B) Produce multiple batches of blue jeans at several different temperatures but with the same enzyme concentration and then compare how the lightness of the blue jeans varies between batches.
C) Produce multiple batches of blue jeans at several different temperatures and with different enzyme concentrations and then compare how the lightness of the blue jeans varies between batches.
D) Produce one batch of blue jeans at a high temperature without the enzyme and another batch of blue jeans at a low temperature with the enzyme and then compare how the lightness of the blue jeans varies between batches.