After DNA fragments with matching sticky ends are temporarily joined by complementary base pairing, the union can be made permanent by the "pasting" enzyme
A) DNA ligase.
B) DNA polymerase.
C) Cas9.
D) reverse transcriptase.

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme that catalyzes the formation of phosphodiester bonds between the adjacent nucleotides of DNA fragments. During DNA cloning, DNA fragments with compatible sticky ends can base pair and form a temporary bond, and this bond is then sealed by the action of DNA ligase, resulting in permanent fusion of the fragments. DNA polymerase is involved in DNA replication, and reverse transcriptase is an enzyme that synthesizes DNA from an RNA template, while Cas9 is an enzyme used in gene editing via the CRISPR-Cas9 system.

After DNA Fragments with Matching Sticky Ends Are Temporarily Joined