Changing a Single Amino Acid in a Protein Consisting of 325

Changing a single amino acid in a protein consisting of 325 amino acids would

A) alter the primary structure of the protein but not its tertiary structure or function.
B) cause the tertiary structure of the protein to unfold.
C) always alter the biological activity or function of the protein.
D) always alter the primary structure of the protein and disrupt its biological activity.
E) always alter the primary structure of the protein, sometimes alter the tertiary structure of the protein, and sometimes affect its biological activity.