A researcher introduces double-stranded RNA into a culture of mammalian cells, and can identify its location or that of its smaller subsections experimentally, using a fluorescent probe.
-In addition, she finds what other evidence of this single-stranded RNA piece's activity?

Question

Quizplus · Accepted Answer

The introduction of double-stranded RNA can activate the RNA interference pathway, which leads to the degradation of the target mRNA. This, in turn, can result in a reduced rate of accumulation of the polypeptide encoded by the target mRNA. Therefore, choice C is the best answer. Choice A is incorrect because measuring the degradation rate of the remaining single strand would not provide evidence of the activity of the double-stranded RNA. Choice B is incorrect because measuring the decrease in the concentration of Dicer would not necessarily provide evidence of the activity of the double-stranded RNA. Choice D is incorrect because miRNA is not mentioned in the question stem. Choice E is incorrect because the cell's translation ability would not be entirely shut down by the introduction of double-stranded RNA.

A Researcher Introduces Double-Stranded RNA into a Culture of Mammalian