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Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome.
-A researcher introduces double-stranded RNA into a culture of mammalian cells. The double-stranded RNA corresponds to a region of a protein-coding gene of interest. How may the introduced RNA affect gene expression in these cells?

Question

Quizplus · Accepted Answer

Introducing double-stranded RNA corresponding to a region of a protein-coding gene of interest may trigger a process called RNA interference (RNAi). RNAi is a mechanism for gene silencing that works post-transcriptionally by degrading the mRNA molecule. As a consequence, the amount of protein produced by the target gene may be significantly reduced. The overall rate of transcription and translation in the cell may not be affected. The amount of mRNA produced by the target gene would be reduced, not increased, by RNAi.

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