You are preparing to perform DNA fingerprinting by PCR for the first time. Select the reagent that would result in complications in the interpretation of your results.
A) PCR primers that anneal to the repetitive region of the microsatellites
B) PCR primers that anneal to regions flanking the microsatellites
C) Human DNA
D) Taq polymerase

Question

Quizplus · Accepted Answer

PCR primers that anneal to the repetitive region of the microsatellites would result in complications because they could produce a complex pattern of amplification products due to the variability in the number of repeats within the microsatellites, making interpretation difficult. Primers are ideally designed to anneal to sequences flanking the target region to ensure consistent amplification of the region of interest.

You Are Preparing to Perform DNA Fingerprinting by PCR for the First