In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by ________.
A) net charge
B) molecular mass
C) abundance
D) isoelectric point

Question

Quizplus · Accepted Answer

In a two-dimensional gel electrophoresis experiment, proteins are first separated based on their isoelectric point (pI) using isoelectric focusing (IEF), which separates the proteins vertically according to their net charge. The proteins are then separated horizontally based on their molecular mass using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Therefore, the correct answer is B - proteins are separated vertically based on their isoelectric point and horizontally by their molecular mass.

In a Two-Dimensional Gel Electrophoresis Experiment, Proteins Are Generally Separated