What would be the most reasonable technique to use to determine if a drug is capable of inhibiting a transcription factor?
A) ChIP assay used with a DNA microarray
B) Mass spectrometry of proteins in the cell
C) 2-D gel electrophoresis
D) Antibody microarray

Question

Quizplus · Accepted Answer

A ChIP assay (chromatin immunoprecipitation) can be used to determine if a drug is capable of inhibiting a transcription factor. This technique involves crosslinking the transcription factor to DNA, immunoprecipitating the transcription factor with an antibody, and then quantifying the amount of DNA that has been pulled down with the transcription factor. This quantification can be done using a DNA microarray, which will allow for the detection of changes in the amount of DNA pulled down in response to the drug treatment. Mass spectrometry, 2-D gel electrophoresis, and antibody microarrays may be useful in other contexts, but are not the most reasonable techniques for determining if a drug is capable of inhibiting a transcription factor.

What Would Be the Most Reasonable Technique to Use to Determine