What is the purpose of DNA ligase in a cloning experiment?
A) The purpose is to proofread the vector for mistakes.
B) The purpose is to reestablish the stable sugar-phosphate backbone of the DNA molecule.
C) The purpose is to allow the vector to enter into the cell.
D) The purpose is to function as a selectable marker.

Question

Quizplus · Accepted Answer

DNA ligase is an enzyme that catalyzes the formation of phosphodiester bonds between adjacent nucleotides in DNA, which helps reestablish the stable sugar-phosphate backbone of the DNA molecule. In a cloning experiment, DNA ligase is used to join together (ligate) the vector (usually a plasmid) and the foreign DNA fragment (insert) into a recombinant DNA molecule.

What Is the Purpose of DNA Ligase in a Cloning