What is the purpose of RNaseH in the making of cDNA?
A) It copies the mRNA into a complementary DNA strand.
B) It reforms the sugar-phosphate backbone.
C) It generates short RNAs that are used as primers.
D) It proofreads the cDNA for errors.

Question

Quizplus · Accepted Answer

RNaseH degrades the RNA strand of an RNA-DNA hybrid, allowing the remaining RNA fragments to serve as primers for DNA polymerase to synthesize the second DNA strand.

What Is the Purpose of RNaseH in the Making of CDNA