In cancer cells one allele of the tumor suppressor gene p53 is frequently mutated so that the protein is inactive, not produced, or deleted. The other allele will usually have a normal sequence and the promoter remains intact but the gene is not expressed. Sequencing with sodium bisulfite modification of DNA can be used to detect which cytosines are methylated. If the cancer cell DNA is sequenced what would be the anticipated results?

Question

Quizplus · Accepted Answer

Mutations in the p53 tumor suppressor gene in cancer cells can lead to the methylation of cytosines in or near the promoter region. This methylation can silence the expression of the remaining normal allele, further contributing to the development and progression of cancer. Therefore, the anticipated results of sequencing with sodium bisulfite modification of DNA in a cancer cell would be the methylation of cytosines in or near the promoter region of the p53 gene.

In Cancer Cells One Allele of the Tumor Suppressor Gene