You are performing a biochemical purification of enzymes involved in DNA replication. You have purified the replisome. You wish to purify the primosome. You perform further separation techniques on your purified replisome. How will you test to determine that you have purified the primosome?
A) You should confirm that your sample has helicase and primase activity, but not the ability to synthesize DNA.
B) You should confirm that your sample has the ability to synthesize DNA and has primase activity, but no helicase activity.
C) You should confirm that your sample has helicase and primase activity. This is the only test that is needed.
D) You should confirm that your sample has primase activity and can synthesize DNA. This is the only test that is needed.

Question

Quizplus · Accepted Answer

The primosome is a protein complex essential for DNA replication that includes both helicase, which unwinds the DNA helix, and primase, which synthesizes RNA primers. It does not have the ability to synthesize DNA, which is a function of DNA polymerases within the replisome. Therefore, confirming the presence of helicase and primase activity without DNA synthesis capability indicates successful primosome purification.

You Are Performing a Biochemical Purification of Enzymes Involved in DNA