You perform comparative genomic hybridization. You correctly synthesize your red and green DNA, but you forget to treat the DNA with heat before you apply the samples to metaphase chromosomes. What will the ratio of green fluorescence to red fluorescence be?
A) 0
B) 1
C) 2
D) There will be no signal.

Question

Quizplus · Accepted Answer

Without the heat treatment, the DNA strands will not denature and will not hybridize with the complementary probes. Therefore, there will be no hybridization and no signal.

You Perform Comparative Genomic Hybridization