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Joshua Lederberg and Edward Tatum Conducted Experiments with Two Auxotrophic

Question 23

Multiple Choice

Joshua Lederberg and Edward Tatum conducted experiments with two auxotrophic strains of E.coli.The Y20 strain had the genotype thr- leu- thi- bio+ phe+ cys+, and the Y24 strain had the genotype thr+ leu+ thi+ bio- phe- cys-.Which of the following procedures would produce growth of bacterial colonies?


A) Spread the Y20 strain on minimal medium agar plates.
B) Spread the Y24 strain on minimal medium agar plates.
C) Boil the Y20 strain, and spread it on minimal medium agar plates.
D) Boil the Y24 strain, and spread it on minimal medium agar plates.
E) Mix the Y20 and Y24 strains together, and spread it on minimal medium agar plates.

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