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You Are Studying a New Phage That Infects H

Question 45

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You are studying a new phage that infects H.pylori.You isolated two mutant strains of the phage, each producing a different plaque phenotype due to a specific mutation: rough (r)and big (b).You coinfect H.pylori with both strains by adding a mixture of phages to a culture of cells.You collect the cell lysate containing progeny phages; plate diluted phages on a lawn of H.pylori cells; and observe 970 rough plaques, 890 big plaques, 0 rough and big plaques, and 500 normal, wild-type plaques.
a.What is the recombination frequency between the r locus and the b locus?
b.Can r and b be different alleles of the same locus?
c.How can you explain the results?

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b.No, the 500 wild-type plaque...

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