Which of the following is NOT true regarding the basic components required for a bacterial cloning vector?
A) Selectable markers provide a means for preferentially allowing growth of only those bacterial cells that have been transformed with the cloning vector.
B) Unique restriction enzyme sites allow for pieces of foreign DNA to be inserted into the bacterial plasmid cloning vector that range in size from 20 kb to 50 kb.
C) Unique restriction enzyme sites provide a means for inserting the foreign DNA into the cloning vector at a specific, known, sequence site.
D) A bacterial origin of replication ensures that the plasmid is replicated while present within the bacterial cell.
E) Selectable markers provide a means for selecting cells that have been transformed with a recombinant plasmid.
Correct Answer:
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