Before sequencing, the DNA fragment was cloned into a plasmid.On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was CLOSEST to the primer?

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The Answer of Before sequencing, the DNA fragment was cloned into a plasmid.On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was CLOSEST to the primer?

Before Sequencing, the DNA Fragment Was Cloned into a Plasmid