You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA.Southern blot analysis shows that the probe detects a 2000 bp fragment in one strain of mice and a 4-kb fragment in another strain of mice.The two mice strains are crossed to produce an F₁ generation.Two F₁ siblings are mated to produce a dozen F₂ progeny.You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe. How many bands would be seen in the Southern blot of an F₁ individual?

Question

Quizplus · Accepted Answer

In the F₁ individual, there would be either one band of 2000 bp or one band of 4-kb fragment due to the presence of a single allele inherited from either parent. Therefore, the Southern blot of an F₁ individual would show two bands, one for each allele inherited from each parent.

You Identify an RFLP in Mice by Digesting Genomic DNA