The genome of Mycobacterium tuberculosis consists of a single circular chromosome that is 4, 410, 000 nucleotides in length and has a 66% (2/3)GC content.The MseI restriction enzyme cuts at a 5' TTAA 3' sequence (one DNA strand given).How many MseI sites should be present in this genome?

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Quizplus · Accepted Answer

The MseI restriction enzyme cuts at a 5F1F1F1S1F1F1F10 TTAA 3F1F1F1S1F1F1F10 sequence (one DNA strand given). The GC content of the genome is 66% (2/3). So, the AT content is 33% (1/3). The frequency of TTAA in the genome is (1/3) x (1/3) x (1/3) x (1/3) = 1/81. The length of the genome is 4, 410, 000 nucleotides. So, the number of MseI sites in the genome is 4, 410, 000 / 81 = 54, 444.

The Genome of Mycobacterium Tuberculosis Consists of a Single Circular