An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem?
A) Histones that were on the DNA when it was isolated from E. coli are blocking access to the template.
B) There is a mutation in the inverted repeat sequence that prevents a hairpin secondary structure from forming.
C) There is a mutation at -10, where a promoter consensus sequence is located.
D) Rho factor has not been added.
E) TATA-binding protein (TBP) has not been added.

Question

Quizplus · Accepted Answer

The -10 region is part of the promoter in bacterial genes, and a mutation here can prevent RNA polymerase from recognizing and binding to the promoter, thus inhibiting transcription initiation.

An in Vitro Transcription System That Contains a Bacterial Gene