Which Is Not One of the Elements Needed to Amplify

Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR) ?

A) two primers-short polynucleotides that are complementary to the two ends of the segment to be amplified
B) a restriction endonuclease enzyme that cleaves DNA at specific locations
C) the segment of DNA that must be copied
D) a DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of DNA from a template strand
E) nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA