Suppose There Is (Hypothetical)evidence That a Gene in Mice Produces

Suppose there is (hypothetical) evidence that a gene in mice produces a substance that induces twitchiness in hind leg muscles. A female mouse of a true-breeding twitchy strain is mated with a male of a true-breeding non-twitchy strain. All progeny are twitchy. Which of these procedures would you use to provide evidence for your hypothesis that twitchiness is due to mtDNA?

A) Sequence the mtDNA of the twitchy mother.
B) Breed twitchy male progeny with normal females to show no paternal inheritance.
C) Breed twitchy female with twitchy male F1 mice to verify there is maternal inheritance.
D) Backcross twitchy female offspring with normal fathers.
E) Sequence the mtDNA of the twitchy male offspring.