In selecting recombinant bacteria, cells are chosen that are resistant to a specific antibiotic. How are the bacteria made resistant?
A)They are pre-selected for the experiment on this basis.
B)The antibiotic resistance gene is encoded in the vector.
C)The antibiotic resistance gene is encoded on the donor insert.
D)Resistance is activated by the recombination event.
E)Resistance is activated when the cells are provided with the antibiotic

Question

Quizplus · Accepted Answer

The antibiotic resistance gene is encoded in the vector, which is used to introduce the donor insert into the recipient bacterium. The vector typically contains a selectable marker, such as an antibiotic resistance gene, that allows for the identification and selection of successfully transformed cells. Therefore, the bacteria are made resistant to the specific antibiotic by acquiring the antibiotic resistance gene from the vector during the transformation process.

In Selecting Recombinant Bacteria, Cells Are Chosen That Are Resistant