In reverse genetics, what is the correct order in which the experimenter proceeds?
A) silencing the genes in question using RNAi, followed by generating gain-of-function alleles
B) screening genes via PCR, followed by deletion of the flanking sequences
C) selection of a phenotypic alternative for the gene in question and sequencing its DNA
D) induction of and selection for a mutant allele with a known sequence, followed by screening for mutations of interest
E) random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits
Correct Answer:
Verified
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A)it breaks
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Q22: In a given situation, reduced or missing
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