Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of an oncogene in vitro?

Question

Quizplus · Accepted Answer

RNA interference (RNAi) is a molecular technique used to reduce expression of specific genes, including oncogenes. It involves introducing short small interfering RNAs (siRNAs) that are complementary to the mRNA of the target gene, causing mRNA degradation and subsequent reduction of protein production. PCR, DNAse sensitivity assay, Southern blotting, and western blotting are not effective methods for reducing gene expression.

Many Types of Cancer Are Known to Overexpress the Receptor