You have isolated DNA for PCR and accidentally added topoisomerase instead of polymerase to your sample. What would you expect if you ran this DNA sample on a gel?
A)The DNA would show increased supercoiling and have a lower electrophoretic mobility.
B)The DNA would show decreased supercoiling and have a higher electrophoretic mobility.
C)The DNA would show increased supercoiling and have a higher electrophoretic mobility.
D)The DNA would show decreased supercoiling and have a lower electrophoretic mobility.
E)The DNA would show decreased supercoiling, but electrophoretic mobility will be unaffected.

Question

Quizplus · Accepted Answer

The Answer of You have isolated DNA for PCR and accidentally added topoisomerase instead of polymerase to your sample. What would you expect if you ran this DNA sample on a gel?
A)The DNA would show increased supercoiling and have a lower electrophoretic mobility.
B)The DNA would show decreased supercoiling and have a higher electrophoretic mobility.
C)The DNA would show increased supercoiling and have a higher electrophoretic mobility.
D)The DNA would show decreased supercoiling and have a lower electrophoretic mobility.
E)The DNA would show decreased supercoiling, but electrophoretic mobility will be unaffected.

You Have Isolated DNA for PCR and Accidentally Added Topoisomerase