You are trying to prepare a DNA karyotype, but this time you forgot to add trypsin to your chromosome preparation. What effect would you expect to see?
A)Dyes would stain the chromosomes darker because more proteins would be present to take up the dye.
B)Dyes would not stain the chromosomes as expected because proteins would not be removed from the chromatin and so would block the dye from accessing the DNA.
C)Chromosomes would not condense properly, so it would be very difficult to isolate individual chromosomes.
D)Chromosomes would be degraded quickly because the trypsin is necessary to promote DNA stability by adding more scaffold proteins to the chromatin.
E)The chromosomes would be more difficult to pair up because the trypsin acts as a dye, creating a banding pattern that distinguishes between chromosomes.

Question

Quizplus · Accepted Answer

Trypsin is an enzyme that is often used to digest proteins from a sample in order to facilitate chromosome staining. Without trypsin, the proteins would not be removed from the chromatin, and the dyes used to stain the chromosomes would not be able to access the DNA effectively. This would result in a weaker or incomplete staining pattern.

You Are Trying to Prepare a DNA Karyotype, but This