We can detect the SNP in the β^S allele because it destroys a DdeI restriction site, leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the
β-globin gene, depending on the allele. One DdeI restriction sequence is eliminated from the β^S allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG, while a newly identified SNP is CCTTAG. Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

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The Answer of We can detect the SNP in the β^S allele because it destroys a DdeI restriction site, leading to DNA restriction fragment variation revealed by Southern blot analysis. Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the β-globin gene, depending on the allele. One DdeI restriction sequence is eliminated from the β^S allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′. Suppose the wild-type allele sequence is CCTGAG, while a newly identified SNP is CCTTAG. Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

We Can Detect the SNP in the βS Allele Because

We Can Detect the SNP in the β^S Allele Because