The PCR reaction mixture does NOT include:
A) all four deoxynucleoside triphosphates.
B) DNA containing the sequence to be amplified.
C) DNA ligase.
D) heat-stable DNA polymerase.
E) oligonucleotide primer(s).

Question

Quizplus · Accepted Answer

The PCR reaction mixture includes all four deoxynucleoside triphosphates, DNA containing the sequence to be amplified, heat-stable DNA polymerase, and oligonucleotide primer(s). However, it does not include DNA ligase, as PCR uses a different mechanism for amplification than the DNA replication process that requires DNA ligase.

The PCR Reaction Mixture Does NOT Include