If you want to clone a gene by using E. coli and then transfer that cloned gene into yeast to express the protein the gene encodes, what type of self-replicating DNA should you use for your cloning process?
A) a bacmid
B) a plasmid
C) a yeast artificial chromosome
D) a shuttle vector
E) a bacterial artificial chromosome

Question

Quizplus · Accepted Answer

Shuttle vectors are ideal for transferring genetic material between different organisms, since they contain the necessary elements for replication in both bacterial and eukaryotic cells. In this case, the gene would be cloned into a shuttle vector that can replicate in E. coli and then be transferred into yeast for expression. Bacmids and bacterial artificial chromosomes (BACs) are specifically designed for cloning in bacteria, while yeast artificial chromosomes (YACs) are designed for cloning in yeast, and may not be compatible with both organisms. Plasmids, while commonly used for cloning, would need to be modified with additional regulatory elements to allow for expression in yeast.

If You Want to Clone a Gene by Using E