A good expression vector for protein expression in E. coli should have which feature(s)?
A) an origin of replication
B) a selectable marker
C) a promoter, operator, and ribosome-binding site
D) All of the answers are correct.
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

A good expression vector for protein expression in E. coli should have all of the features mentioned in options A, B, and C. An origin of replication is necessary to ensure the plasmid is stably maintained in E. coli, a selectable marker allows for the selection of bacteria that have taken up the plasmid, and a promoter, operator, and ribosome-binding site are essential for the efficient expression of the protein of interest. Therefore, all the given answers are correct.

A Good Expression Vector for Protein Expression in E