Which technique can be used to estimate the relative copy numbers of particular DNA sequences in a sample?
A) reverse transcriptase PCR
B) quantitative PCR
C) some gel based analysis such as with the Bioanalyzer and Fragment Analyzer gel band density are used in calculating concentration in a lot of labs.
D) normal PCR
E) in vitro transcription

Question

Quizplus · Accepted Answer

Quantitative PCR (qPCR) is a well-established technique for estimating the relative copy numbers of particular DNA sequences in a sample. It allows for the detection and quantification of DNA in real-time, providing a dynamic range of detection and accurate quantification of target DNA. Other techniques such as gel-based analysis can be used to estimate DNA concentration, but they are less accurate and have a lower dynamic range compared to qPCR. Reverse transcriptase PCR (RT-PCR) and in vitro transcription are techniques used for measuring RNA expression levels rather than DNA copy numbers. Normal PCR is not quantitative and does not provide accurate quantification of copy numbers.

Which Technique Can Be Used to Estimate the Relative Copy