If you want to determine what other proteins might be interacting with the protein you have been studying, what type of fusion tag would you add to your protein to aid in purification of both the protein you have been studying and the proteins interacting with it?
A) a green fluorescent protein fusion tag
B) a tandem affinity purification tag
C) a maltose-binding protein tag
D) an epitope tag
E) a $\beta$-galactosidase tag

Question

Quizplus · Accepted Answer

A tandem affinity purification tag, such as the commonly used FLAG-HA tag, allows for the purification of the protein of interest and any interacting proteins through two rounds of purification steps, increasing the specificity of the purification process. This tag allows for the identification of protein-protein interactions and subsequent analysis. The other options, including fluorescent and enzymatic tags, do not aid in purification for protein-protein interaction analysis.

If You Want to Determine What Other Proteins Might Be β\betaβ

If You Want to Determine What Other Proteins Might Be $\beta$