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In the Chemical Synthesis of DNA

Question 27

Multiple Choice

In the chemical synthesis of DNA:


A) the dimethoxytrityl (DMT) group catalyzes formation of the phosphodiester bond.
B) the direction of synthesis is 5' to 3'.
C) the maximum length of oligonucleotide that can be synthesized is 8-10 nucleotides.
D) the nucleotide initially attached to the silica gel support will become the 3' end of the finished product.
E) the protecting cyanoethyl groups are removed after each step.

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