Enzymatic hydrolysis of a purified polysaccharide with a glycosidase that cleaves specifically usually occurs before analysis by:

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Enzymatic hydrolysis with a glycosidase or other enzyme is likely to release smaller carbohydrate units that can be analyzed by NMR (nuclear magnetic resonance) or mass spectrometry. These techniques are commonly used for carbohydrate structure elucidation. Ion-exchange, gel-filtration, and lectin-affinity chromatography are separation techniques that can be used to analyze or purify specific carbohydrates, but they may not be sensitive enough to detect small changes in structure or composition that can be revealed by NMR or mass spectrometry. Gas-liquid chromatography is typically used for volatile or derivatized compounds and may not be suitable for analysis of large polysaccharides.

Enzymatic Hydrolysis of a Purified Polysaccharide with a Glycosidase That