The Lineweaver-Burk plot is used to:
A) determine the equilibrium constant for an enzymatic reaction.
B) extrapolate for the value of reaction rate at infinite enzyme concentration.
C) illustrate the effect of temperature on an enzymatic reaction.
D) solve, graphically, for the rate of an enzymatic reaction at infinite substrate concentration.
E) solve, graphically, for the ratio of products to reactants for any starting substrate concentration.

Question

Quizplus · Accepted Answer

The Lineweaver-Burk plot is a graphical representation used to determine the kinetic parameters of an enzyme-catalyzed reaction. It involves plotting the inverse of the initial reaction rate against the inverse of substrate concentration. The resulting plot is a straight line with a y-intercept equal to 1/Vmax and an x-intercept equal to -1/Km. By extrapolating the line to the y-axis, the value for 1/Vmax can be obtained, which represents the reaction rate at infinite enzyme concentration. Therefore, option D, "solve, graphically, for the rate of an enzymatic reaction at infinite substrate concentration," is the best choice.

The Lineweaver-Burk Plot Is Used To