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The Double-Reciprocal Transformation of the Michaelis-Menten Equation, Also Called the Lineweaver-Burk

Question 21

Multiple Choice

The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by 1/V0 = Km /(Vmax[S]) + 1/Vmax
To determine Km from a double-reciprocal plot, you would:


A) multiply the reciprocal of the x-axis intercept by -1.
B) multiply the reciprocal of the y-axis intercept by -1.
C) take the reciprocal of the x-axis intercept.
D) take the reciprocal of the y-axis intercept.
E) take the x-axis intercept, where V0 = 1/2 Vmax.

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