Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme. This is an example of what kind of inhibition?

Question

Quizplus · Accepted Answer

PMSF binds covalently to the catalytic serine residue at the active site, irreversibly inhibiting the enzyme. This inactivation is not reversible by simply removing the inhibitor, and the enzyme-inhibitor bond is not cleaved by the enzyme. Therefore, this is an example of irreversible inhibition.

Phenyl-Methane-Sulfonyl-Fluoride (PMSF) Inactivates Serine Proteases by Binding Covalently to the Catalytic