In what way does an uncompetitive inhibitor bind to an enzyme?
A) It reversibly binds to the enzyme active site.
B) It irreversibly binds to the enzyme active site.
C) It reversibly binds to the enzyme-substrate complex but does not bind to the free enzyme.
D) It reversibly binds to both the free enzyme and the enzyme-substrate complex.
E) It irreversibly binds to an enzyme allosteric site.

Question

Quizplus · Accepted Answer

Uncompetitive inhibitors specifically bind to the enzyme-substrate complex, not to the free enzyme, and they do so reversibly. This binding typically occurs at a site distinct from the active site, which is why they do not bind to the free enzyme.

In What Way Does an Uncompetitive Inhibitor Bind to an Enzyme