An enzyme catalyzes the reaction A B. The initial rate of the reaction was measured as a function of the concentration of A. The following data were obtained:
[A], micromolar
V0, nmoles/min
0.05
0.08
0.1
0.16
0.5
0.79
1
1.6
5
7.3
10
13
50
40
100
53
500
73
1,000
76
5,000
79
10,000
80
20,000
80
a) What is the Km of the enzyme for the substrate A?
b) What is the value of V0 when [A] = 43?
The above data were plotted as 1/V0 versus 1/[A], and a straight line was obtained.
c) What is the value of the y-intercept of the line?
d) What is the value of the x-intercept of the line?
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