You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained:
[S]
V₀
-Inhibitor
+Inhibitor
0.0001
33
17
0.0002
50
29
0.0005
71
50
0.001
83
67
0.002
91
80
0.005
96
91
0.01
98
95
0.02
99
98
0.05
100
99
0.1
100
100
0.2
100
100
a) What is the V_max in the absence of inhibitor?
b) What is the K_m in the absence of inhibitor?
c) When [S] = 0.0004, what will V₀ be in the absence of inhibitor?
d) When [S] = 0.0004, what will V₀ be in the presence of inhibitor?
e) What kind of inhibitor is it likely to be?

Question

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The Answer of You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] V₀ -Inhibitor +Inhibitor 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.2 100 100 a) What is the V_max in the absence of inhibitor? b) What is the K_m in the absence of inhibitor? c) When [S] = 0.0004, what will V₀ be in the absence of inhibitor? d) When [S] = 0.0004, what will V₀ be in the presence of inhibitor? e) What kind of inhibitor is it likely to be?

You Measure the Initial Rate of an Enzyme Reaction as a Function