Which method would be BEST to use to monitor protein secondary structure during the titration of a denaturing agent?
A) x-ray crystallography
B) electron microscopy
C) circular dichroism
D) mass spectrometry
E) liquid chromatography

Question

Quizplus · Accepted Answer

Circular dichroism is a spectroscopic method that can be used to monitor changes in protein secondary structure. It is sensitive to changes in the helix and sheet proportion of proteins, and is often used to examine protein folding and stability, as well as denaturation by agents such as urea or guanidine hydrochloride. X-ray crystallography and electron microscopy are typically used to determine protein structure at higher resolution, but they are not well-suited for real-time monitoring of structural changes. Mass spectrometry and liquid chromatography can provide information on protein composition and modifications, but are not as sensitive to changes in secondary structure.

Which Method Would Be BEST to Use to Monitor Protein