The protein bovine serum albumin (BSA), has a MW of about 66,400. Using the Lambert-Beer Law, calculate the concentration of a sample of BSA with an absorbance at 280 nm of 1.3, assuming the molar extinction coefficient at 280 nm is 43,824 M^-¹cm^-¹.

Question

Quizplus · Accepted Answer

Using the Lambert-Beer Law, A = εcl, where A is absorbance, ε is the molar extinction coefficient, c is concentration, and l is path length (1 cm). Solving for c: c = A/ε = 1.3 / 43,824 = 2.97 x 10^-5 M. Converting to mg/mL: (2.97 x 10^-5 M) x (66,400 g/mol) x (1000 mg/g) = 1.97 mg/mL.

The Protein Bovine Serum Albumin (BSA), Has a MW of About