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The Following Reagents Are Often Used in Protein Chemistry

Question 104

Essay

The following reagents are often used in protein chemistry. Match the reagent with the purpose for which it is best suited. Some answers may be used more than once or not at all; more than one reagent may be suitable for a given purpose.
(a) CNBr (cyanogen bromide)
(e) performic acid
(b) Edman reagent (phenylisothiocyanate)
(f) chymotrypsin
(c) Sanger reagent (FDNB)
(g) trypsin
(d) reducing agent (dithiothreitol)
(h) iodoacetamide
___ hydrolysis of peptide bonds on the carboxyl side of Lys and Arg
___ cleavage of peptide bonds on the carboxyl side of Met
___ breakage of disulfide (-S-S-) bonds
___ modification of sulfhydryl groups of Cys
___ determination of the amino acid sequence of a peptide
___ determining the amino-terminal amino acid in a polypeptide

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